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n-heptane 4

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Serum Nem>-Glycan Markers for Diagnosing Significant Liver Fibrosis and Cirrhosis in Chronic Hepatitis Article

Lin Wang, Yiqi Liu, Qixin Gu, Chi Zhang, Lei Xu, Lei Wang, Cuiying Chen, Xueen Liu, Hong Zhao, Hui Zhuang

Engineering 2023, Volume 26, Issue 7, Pages 151-158 doi: 10.1016/j.eng.2023.03.008

Abstract: Serum Nem>-glycan profiles were tested using DNA sequencer-assisted fluorophore-assisted carbohydrateA total of nine Nem>-glycan peaks were identified in serum samples for each subject.In distinguishing significant liver fibrosis, the diagnostic efficiency of the serum Nem>-glycanIn distinguishing liver cirrhosis, the diagnostic AUROC curve of the serum Nem>-glycan RF modelThe diagnostic efficiency of the constructed serum Nem>-glycan models (RF-A and RF-B) was superior

Keywords： Liver fibrosis Chronic hepatitis B Serum Nem>-glycan Nem>-glycan model Alanine aminotransferase

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Removable Dyes—The Missing Link for In-Depth Nem>-Glycan Analysis via Multi-Method Approaches Article

Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp

Engineering 2023, Volume 26, Issue 7, Pages 132-150 doi: https://doi.org/10.1016/j.eng.2023.02.016

Abstract: To fully delineate glycan structures, we developed an integrative approach combining a set of cost-effectiveThrough the detailed structural analysis of selected, dauntingly complex Nem>-glycans from chickenovalbumin, horse serum, and bovine transferrin, we illustrate the capabilities of the presented strategyMoreover, this approach "visualizes" Nem>-glycans that have been difficult to identifyfar—such as the sulfated glycans on human immunoglobulin A—including minute changes in glycan

Keywords： Glycoproteins N-glycans Reversible labeling Hydrophilic interaction liquid chromatography Capillary

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Novel Insight into the Etiology of Haff Disease by Mapping the Nem>-Glycome with Orthogonal Mass Article

Si Liu, Yuanyuan Liu, Jiajing Lin, Bi-Feng Liu, Zhenyu He, Xiaomin Wu, Xin Liu

Engineering 2023, Volume 26, Issue 7, Pages 63-73 doi: 10.1016/j.eng.2022.09.012

Abstract: Profiling of the Nem>-glycome in HD would assist in deciphering the underlying molecular mechanismHerein, we enrolled 90 serum samples with HD patients and healthy controls from the Wuhan Center forNem>-glycome profiles of both serum and serum-derived immunoglobulin G (IgG) in HD were characterizedThe serum level of IgG was found to serve as a good indicator for HD patients.Our study reveals the association of differential IgG N-glycosylation with HD, providing new insight

Keywords： Haff disease Serum Immunoglobulin G Glycosylation Disease pathogenesis

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Profound Diversity of the Nem>-Glycome from Microdissected Regions of Colorectal Cancer, Stroma Article

Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer

Engineering 2023, Volume 26, Issue 7, Pages 32-43 doi: 10.1016/j.eng.2022.08.016

Abstract: While the Nem>-glycosylation of cell lines and serum has been widely examined, the analysis ofcancer-associated Nem>-glycans from tissues has been hampered by the heterogeneity of tumors andthe complexity of Nem>-glycan structures.Nem>-glycan alditols were analyzed by means of porous graphitized carbon liquid chromatography-electrospray>Nem>-glycans, as well as a decrease in bisected Nem>-glycans.

Keywords： Colorectal cancer Tumor Porous graphitized carbon liquid chromatography mass spectrometry N-glycomics

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High-Throughput Profiling of Serological Immunoglobulin G Nem>-Glycome as a Noninvasive Biomarker Article

Pengcheng Liu, Xiaobing Wang, Aishe Dun, Yutong Li, Houqiang Li, Lu Wang, Yichun Zhang, Cancan Li, Jinxia Zhang, Xiaoyu Zhang, Lixing Ma, Haifeng Hou

Engineering 2023, Volume 26, Issue 7, Pages 44-53 doi: 10.1016/j.eng.2023.02.008

Abstract: >Nem>-glycome.A Lasso algorithm, accompanied by a logistic regression model, was used to develop a glycan-based modelIn addition, the composition of IgG Nem>-glycans was correlated with that of inflammatory cytokines(rem> = 0.556).The glycan-based models for diagnosing GI cancers exhibited an excellent performance, with areas under

Keywords： Gastrointestinal cancer Glycosylation Immunoglobulin G Diagnostic biomarker

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Topology optimization of piezoelectric bi-material actuators with velocity feedback control

Mariana MORETTI, Emílio C. N. SILVA

Frontiers of Mechanical Engineering 2019, Volume 14, Issue 2, Pages 190-200 doi: 10.1007/s11465-019-0537-y

Abstract: In recent years, the new technologies and discoveries on manufacturing materials have encouraged researchers to investigate the appearance of material properties that are not naturally available. Materials featuring a specific stiffness, or structures that combine non-structural and structural functions are applied in the aerospace, electronics and medical industry fields. Particularly, structures designed for dynamic actuation with reduced vibration response are the focus of this work. The bi-material and multifunctional concepts are considered for the design of a controlled piezoelectric actuator with vibration suppression by means of the topology optimization method (TOM). The bi-material piezoelectric actuator (BPEA) has its metallic host layer designed by the TOM, which defines the structural function, and the electric function is given by two piezo-ceramic layers that act as a sensor and an actuator coupled with a constant gain active velocity feedback control (AVFC). The AVFC, provided by the piezoelectric layers, affects the structural damping of the system through the velocity state variables readings in time domain. The dynamic equation analyzed throughout the optimization procedure is fully elaborated and implemented. The dynamic response for the rectangular four-noded FE analysis is obtained by the Newmark’s time-integration method, which is applied to the physical and the adjoint systems, given that the adjoint formulation is needed for the sensitivity analysis. A gradient-based optimization method is applied to minimize the displacement energy output measured at a predefined degree-of-freedom of the BPEA when a transient mechanical load is applied. Results are obtained for different control gain values to evaluate their influence on the final topology.

Keywords： topology optimization method bi-material piezoactuator active velocity feedback control time-domain transient analysis host structure design vibration suppression

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Human Prostate-Specific Antigen Carries Nem>-Glycans with Ketodeoxynononic Acid Article

Wei Wang, Tao Zhang, Jan Nouta, Peter A. van Veelen, Noortje de Haan, Theo M. de Reijke, Manfred Wuhrer, Guinevere S.M. Lageveen-Kammeijer

Engineering 2023, Volume 26, Issue 7, Pages 119-131 doi: 10.1016/j.eng.2023.02.009

Abstract: Here, we report for the first time that Kdn is expressed on prostate-specific antigen (PSA) Nem

Keywords： Ketodeoxynononic acid Kdn Glycosylation Prostate cancer Prostate-specific antigen

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Temporal and spatial stability of the EM/PM molecular subtypes in adult diffuse glioma

Frontiers of Medicine 2023, Volume 17, Issue 2, Pages 240-262 doi: 10.1007/s11684-022-0936-z

Abstract: After identifying conserved gene modules co-expressed with EGFR or PDGFRA (EM or PM), we recently proposedan EM/PM classification scheme for adult gliomas in a histological subtype- and grade-independent mannerRNA-seq samples, and clinical samples, we here demonstrate the temporal and spatial stability of the EMThe EM and PM subtypes, which progress in a subtype-specific mode, are robustly maintained in pairedWithin individual gliomas, the EM/PM subtype was preserved across regions and single cells.

Keywords： glioma progression molecular classification EM/PM subtyping intratumor heterogeneity

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Flow cytometric assessment of the effects of chlorine, chloramine, and UV on bacteria by using nucleic acid stains and 5-cyano-2,3-ditolyltetrazolium chloride

Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 6, doi: 10.1007/s11783-016-0884-4

Abstract: Flow cytometry based on nucleic acid stains and CTC was established and optimized. Membrane of is more resistant to chlorine/chloramine than . The metabolic activity of bacteria persisted after the cytomembranewas damaged. showed more resistance to UV irradiation than by FCM. MP-UV was a stronger inhibitor of metabolic activity than LP-UV. Flow cytometry (FCM) has been widely used in multi-parametric assessment of cells in various research fields, especially in environmental sciences. This study detected the metabolic activity of and by using an FCM method based on 5-cyano-2,3-ditolyltetrazolium chloride (CTC); the accuracy of this method was enhanced by adding SYTO 9 and 10% R2A broth. The disinfection effects of chlorine, chloramine, and UV were subsequently evaluated by FCM methods. Chlorine demonstrated stronger and faster destructive effects on cytomembrane than chloramine, and nucleic acids decomposed afterwards. The metabolic activity of the bacteria persisted after the cytomembranewas damaged as detected using CTC. Low-pressure (LP) UV or medium-pressure (MP) UV treatments exerted no significant effects on membrane permeability. The metabolic activity of the bacteria decreased with increasing UV dosage, and MP-UV was a stronger inhibitor of metabolic activity than LP-UV. Furthermore, the membrane of Gram-positive was more resistant to chlorine/chloramine than that of Gram-negative . In addition, showed higher resistance to UV irradiation than .

Keywords： Flow cytometry Escherichia coliem> Staphylococcusaureusem> UV CTC SYTO 9

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VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications

Mani Abirami, Krishnan Kannabiran

Frontiers of Chemical Science and Engineering 2016, Volume 10, Issue 4, Pages 542-551 doi: 10.1007/s11705-016-1599-6

Abstract: We present the microbial green synthesis of silver nanoparticles (NPs) by VITHM1 strain (MTCC No. 12465). The secondary metabolites in the cell free supernatant of this bacterium when incubated with 1 mmol/L AgNO , mediated the biological synthesis of AgNPs. The synthesized AgNPs were characterized by UV-visible spectrum, X-ray diffraction (XRD), atomic force microscope, scanning electron microscopy equipped with energy dispersive spectroscopy, transmission electron microscopy, FT-IR spectroscopy, dynamic light scattering and zeta potential. They were highly stable and, spherical in shape with the average size of 30?50 nm. The secondary metabolites involved in the formation of AgNPs were identified gas chromatography-mass spectrography. The 3D structure of the unit cell of the synthesized AgNPs was determined using XRD data base. The synthesized AgNPs exhibited significant antibacterial activity against tested bacterial pathogens, and did not show haemolysis on human red blood cells. This green synthesis could provide a new platform to explore and use AgNPs as antibacterial therapeutic agents.

Keywords： Streptomyces ghanaensisem> VITHM1 nanoparticles 3D structure antibacterial activity

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Enhanced disinfection of Escherichia coliem> and bacteriophage

Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 4, doi: 10.1007/s11783-016-0854-x

Abstract: A novel photocatalytic Ag-Cu-TiO nanowire membrane was fabricated. Bacteria and virus disinfection was improved by co-depositing Ag and Cu onto membrane. Synergetic photocatalytic effects and free metal ions of Ag and Cu contribute to disinfection. 7.68 log removal of and 4.02 log removal of bacteriophage MS2 were achieved. Titanium dioxide (TiO ) is a widely used photocatalyst that has been demonstrated for microorganism disinfection in drinking water. In this study, a new material with a novel structure, silver and copper loaded TiO nanowire membrane (Cu-Ag-TiO ) was prepared and evaluated for its efficiency to inactivate and bacteriophage MS2. Enhanced photo-activated bactericidal and virucidal activities were obtained by the Cu-Ag-TiO membrane than by the TiO , Ag-TiO and Cu-TiO membranes under both dark and UV light illumination. The better performance was attributed to the synergies of enhanced membrane photoactivity by loading silver and copper on the membrane and the synergistic effect between the free silver and copper ions in water. At the end of a 30 min test of dead-end filtration under 254 nm UV irradiation, the Cu-Ag-TiO membrane was able to obtain an removal of 7.68 log and bacteriophage MS2 removal of 4.02 log, which have met the US EPA standard. The free metal ions coming off the membrane have concentrations of less than 10 ppb in the water effluent, far below the US EPA maximum contaminant level for silver and copper ions in drinking water. Therefore, the photo-activated disinfection by the Cu-Ag-TiO membrane is a viable technique for meeting drinking water treatment standards of microbiological water purifiers.

Keywords： Photo-activated disinfection Titanium dioxide Nanowire membrane Silver Copper

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Direct Evidence of Coal Swelling and Shrinkage with Injecting CO₂ and N₂ Using in-situem> Synchrotron X-ray Microtomography Article

Guanglei Zhang, P.G. Ranjith, Herbert E. Huppert

Engineering 2022, Volume 18, Issue 11, Pages 88-95 doi: 10.1016/j.eng.2022.03.010

Abstract: synchrotron X-ray microtomography, we provide the first observational evidence that injecting nitrogen (N2Our findings support the notion that injecting minimally treated flue gas—a mixture of mainly NN₂ makes it possible to maintain a sufficiently high level of coal

Keywords： CCS CO₂ -ECBM Carbon neutrality X-ray imaging Coal permeability

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Isolation and application of predatory

Ran Yu, Shiwen Zhang, Zhoukai Chen, Chuanyang Li

Frontiers of Environmental Science & Engineering 2017, Volume 11, Issue 1, doi: 10.1007/s11783-017-0900-3

Abstract: Indigenous predatory BALO strains were successfully isolated from activated sludge. Sludge SRF and CST were significantly reduced by BALOs induced biolysis process. The increase of BALO input dosage promoted the sludge biolysis efficiency. Sludge biolysis disintegrated flocs and lysed cells for internal water release. The optimal sludge biolysis time was 24 h and no pH adjustment was needed. -and-like organisms (BALOs) are a group of ubiquitous and obligate predatory bacteria and commonly used as biocontrol agents. In this study, an efficient, environmental-friendly, and convenient BALOs encouraged municipal waste sludge biolysis pretreatment technique was developed and investigated for dewaterability enhancement of excess waste sludge. The indigenous predatory BALOs were successfully isolated from the sludge for biolysis treatment. Without any chemical addition or pH adjustment, the sludge specific resistance (SRF) and capillary suction time (CST) were significantly reduced by as high as 53.4% and 23.8%, respectively within 24 h’s treatment, which would further be lowered with the increase of BALOs input dosage. However, the continuous extension of reaction time would worsen the sludge dewaterability. The decreases of SRF and CST accompanied with the increases of sludge disintegration degree and soluble chemical oxygen demand, nitrogen, and phosphorus concentrations all emphasized the contributions of BALOs’ predation activities to sludge disturbance, cell lysis, and consequently the release of sludge intracellular water to finally effectively improve the sludge dewaterability and disposal efficiency.

Keywords： Bdellovibrioem>-and-like organisms (BALOs) Biolysis Activated sludge Dewaterability Predation

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Mercury enrichment in

Chunhao Dai, Pufeng Qin, Zhangwei Wang, Jian Chen, Xianshan Zhang, Si Luo

Frontiers of Environmental Science & Engineering 2017, Volume 11, Issue 1, doi: 10.1007/s11783-017-0892-z

Abstract: Mercury enrichment in response to elevated atmospheric mercury concentrations in the organs of rape ( ) was investigated. Elevated soil mercury content had significant impact on mercury accumulation in rape stems, roots, seeds and seed coats. Leaf mercury in the leaves was mostly affected by atmospheric input while the stems were mostly affected by soil concentrations. Mercury in the aboveground plant tissue mainly derived from atmospheric absorption, and atmospheric mercury absorption in leaves was higher than that in the stems. Mercury enrichment in response to elevated atmospheric mercury concentrations in the organs of rape ( ) was investigated using an open top chamber fumigation experiment and a soil mercury enriched cultivation experiment. Results indicate that the mercury concentration in leaves and stems showed a significant variation under different concentrations of mercury in atmospheric and soil experiments while the concentration of mercury in roots, seeds and seed coats showed no significant variation under different atmospheric mercury concentrations. Using the function relation established by the experiment, results for atmospheric mercury sources in rape field biomass showed that atmospheric sources accounted for at least 81.81% of mercury in rape leaves and 32.29% of mercury in the stems. Therefore, mercury in the aboveground biomass predominantly derives from the absorption of atmospheric mercury.

Keywords： Open top chamber Gaseous elemental mercury (GEM) Soil Mercury Brassica napusem>

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Mapping the structure-activity relationship of

Worawan PANPIPAT, Hasene KESKIN, Zheng GUO

Frontiers of Chemical Science and Engineering 2015, Volume 9, Issue 1, Pages 105-113 doi: 10.1007/s11705-015-1502-x

Abstract: The phase behavior of twelve synthesized -sitosteryl fatty acid esters with acyl moieties with different chain lengths (C2:0-C18:0) and different degrees of unsaturation (C18:1-C18:3) were investigated in pure and mixed Langmuir monolayers with phospholipids. The surface-pressure isotherms showed that short chain -sitosteryl fatty acid esters gave smaller mean molecular areas and had decreased monolayer stability and the long chain steryl esters did not produce collapsed plateaus. All the steryl esters displayed strong condensing effects, but there was a pronounced structural dependency: medium chain esters (C8 and C10) were less efficient than short and long chain esters. Atomic force microscopy imaging demonstrated that monolayers mixed with dipalmitoyl phosphatidylcholine (DPPC) displayed both DPPC-rich and steryl lipid-rich domains. However, the height and area differences between the two phases and the roughness and morphologic patterns were very dependent on the steryl lipid concentrations as well as the length, the degree of unsaturation and the molecular conformations of the acyl segments. These findings not only provide a better understanding of the interactions between phytosteryl hydrophobic derivatives and biomembranes, but also may be of general use for the design and engineering of phytosterol structural derivations for specific food and pharmaceutical applications.

Keywords： βem>-sitosteryl fatty acid esters dipalmitoyl phosphatidylcholine Langmuir monolayer condensing

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Title Author Date Type Operation

Serum Nem>-Glycan Markers for Diagnosing Significant Liver Fibrosis and Cirrhosis in Chronic Hepatitis

Lin Wang, Yiqi Liu, Qixin Gu, Chi Zhang, Lei Xu, Lei Wang, Cuiying Chen, Xueen Liu, Hong Zhao, Hui Zhuang

Journal Article

Removable Dyes—The Missing Link for In-Depth Nem>-Glycan Analysis via Multi-Method Approaches

Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp

Journal Article

Novel Insight into the Etiology of Haff Disease by Mapping the Nem>-Glycome with Orthogonal Mass

Si Liu, Yuanyuan Liu, Jiajing Lin, Bi-Feng Liu, Zhenyu He, Xiaomin Wu, Xin Liu

Journal Article

Profound Diversity of the Nem>-Glycome from Microdissected Regions of Colorectal Cancer, Stroma

Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer

Journal Article

High-Throughput Profiling of Serological Immunoglobulin G Nem>-Glycome as a Noninvasive Biomarker

Pengcheng Liu, Xiaobing Wang, Aishe Dun, Yutong Li, Houqiang Li, Lu Wang, Yichun Zhang, Cancan Li, Jinxia Zhang, Xiaoyu Zhang, Lixing Ma, Haifeng Hou

Journal Article

Topology optimization of piezoelectric bi-material actuators with velocity feedback control

Mariana MORETTI, Emílio C. N. SILVA

Journal Article

Human Prostate-Specific Antigen Carries Nem>-Glycans with Ketodeoxynononic Acid

Wei Wang, Tao Zhang, Jan Nouta, Peter A. van Veelen, Noortje de Haan, Theo M. de Reijke, Manfred Wuhrer, Guinevere S.M. Lageveen-Kammeijer

Journal Article

Temporal and spatial stability of the EM/PM molecular subtypes in adult diffuse glioma

Journal Article

Flow cytometric assessment of the effects of chlorine, chloramine, and UV on bacteria by using nucleic acid stains and 5-cyano-2,3-ditolyltetrazolium chloride

Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao

Journal Article

VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications

Mani Abirami, Krishnan Kannabiran

Journal Article

Enhanced disinfection of Escherichia coliem> and bacteriophage

Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI

Journal Article

Direct Evidence of Coal Swelling and Shrinkage with Injecting CO₂ and N₂ Using in-situem> Synchrotron X-ray Microtomography

Guanglei Zhang, P.G. Ranjith, Herbert E. Huppert

Journal Article

Isolation and application of predatory